Journal: The Journal of Biological Chemistry

Article Title: Temporin-SHf, a New Type of Phe-rich and Hydrophobic Ultrashort Antimicrobial Peptide *

doi: 10.1074/jbc.M109.097204

Figure Lengend Snippet: Antimicrobial and hemolytic activities of temporin-SHf

Article Snippet: TABLE 2 MIC a μ m Gram-negative bacteria E. coli ATCC 25922 25 E. coli ATCC 35218 >200 E. coli D21 100 E. coli ML-35p 30 P. aeruginosa ATCC 27853 >200 Gram-positive bacteria S. aureus ATCC 25923 12.5 E. faecalis ATCC 29212 50 B. megaterium 3 Yeasts C. albicans ATCC 90028 50 C. parapsilosis ATCC 22019 50 S. cerevisiae 12.5 Fungus A. flavus >200 Leishmania protozoan L. infantum promastigotes NA b L. infantum amastigotes NA Human erythrocytes LC 50 a (μ m ) 200 Open in a separate window a MICs and LC 50 (concentration of peptide producing 50% hemolysis) are expressed as average values from three independent experiments performed in triplicate. b NA, not active in the range of 0.94–60 μ m . Antimicrobial and hemolytic activities of temporin-SHf. . Permeabilization/Disruption of the Bacterial Cytoplasmic Membrane Temporin-SHf-induced permeabilization of the bacterial cytoplasmic membrane was revealed by incubating E. coli ML-35p with the peptide (2–100 μ m ) and by measuring the time-dependent hydrolysis of the small chromogenic substrate ONPG into ONP by cytoplasmic β-galactosidase ( ).

Techniques: Bacteria

Journal: The Journal of Biological Chemistry

Article Title: Temporin-SHf, a New Type of Phe-rich and Hydrophobic Ultrashort Antimicrobial Peptide *

doi: 10.1074/jbc.M109.097204

Figure Lengend Snippet: Kinetics of the cytoplasmic membrane leakage of E. coli ML-35p after treatment with increasing concentrations (2, 4, 10, 15, 30, and 100 μm) of temporin-SHf (top) and with 25 μm melittin or 50 μm dermaseptin B2 as positive controls (bottom). The negative control (without peptide) is also shown. The membrane leakage was followed by measuring the hydrolysis of ONPG at 405 nm by the cytoplasmic bacterial β-galactosidase after incubation with the peptides (see “Experimental Procedures”). Data are expressed as the mean ± S.E.

Article Snippet: TABLE 2 MIC a μ m Gram-negative bacteria E. coli ATCC 25922 25 E. coli ATCC 35218 >200 E. coli D21 100 E. coli ML-35p 30 P. aeruginosa ATCC 27853 >200 Gram-positive bacteria S. aureus ATCC 25923 12.5 E. faecalis ATCC 29212 50 B. megaterium 3 Yeasts C. albicans ATCC 90028 50 C. parapsilosis ATCC 22019 50 S. cerevisiae 12.5 Fungus A. flavus >200 Leishmania protozoan L. infantum promastigotes NA b L. infantum amastigotes NA Human erythrocytes LC 50 a (μ m ) 200 Open in a separate window a MICs and LC 50 (concentration of peptide producing 50% hemolysis) are expressed as average values from three independent experiments performed in triplicate. b NA, not active in the range of 0.94–60 μ m . Antimicrobial and hemolytic activities of temporin-SHf. . Permeabilization/Disruption of the Bacterial Cytoplasmic Membrane Temporin-SHf-induced permeabilization of the bacterial cytoplasmic membrane was revealed by incubating E. coli ML-35p with the peptide (2–100 μ m ) and by measuring the time-dependent hydrolysis of the small chromogenic substrate ONPG into ONP by cytoplasmic β-galactosidase ( ).

Techniques: Membrane, Negative Control, Incubation

Journal: Molecules

Article Title: Commercially Available Viola odorata Oil, Chemical Variability and Antimicrobial Activity

doi: 10.3390/molecules28041676

Figure Lengend Snippet: Antimicrobial activity (mg/mL) of essential oils against the selected pathogen reference strains ( n = 3).

Article Snippet: These included reference strain pathogens related to the infections for which V. odorata oil is most commonly recommended, such as respiratory infections ( Klebsiella pneumoniae ATCC 13883, Streptococcus pyogenes ATCC 12344), skin infections ( Pseudomonas aeruginosa ATCC 27858, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25924, Staphylococcus epidermidis ATCC 14990 and Cutibacterium acnes ATCC 6919), opportunistic nosocomial infections ( Acinetobacter baumannii ATCC 17606 and Enterococcus faecium ATCC 8739) and a fungal pathogen reference strain ( Candida albicans ATCC 10231).

Techniques: Activity Assay, Positive Control, Negative Control, Control